Composite

Part:BBa_K1676098:Design

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-18)


Mutant 1 of Lactate Permease Expression Part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1019


Design Notes

Primers were designed to have the start and stop codon so that they will not be mutated during PCR.


Source

Wild type LDP was obtained from a Pseudomonas Strain and random mutagenesis was carried out by PCR to make lots of Mutant LDP. PCR fragments carrying the mutants were then ligated with pLac, RBS and transcription terminator to make a complete expression construct.

References