Composite
Part:BBa_K1676098:Design
Designed by: Ooi Kean Hean Group: iGEM15_NTU-Singapore (2015-09-18)
Mutant 1 of Lactate Permease Expression Part
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1019
Design Notes
Primers were designed to have the start and stop codon so that they will not be mutated during PCR.
Source
Wild type LDP was obtained from a Pseudomonas Strain and random mutagenesis was carried out by PCR to make lots of Mutant LDP. PCR fragments carrying the mutants were then ligated with pLac, RBS and transcription terminator to make a complete expression construct.